Cultivo y fusion de protoplastos []. Loyola Vargas, V.M. Rosell, C.H. (ed.) Villalobos Arambula, V.M. (ed.) Access the full text: NOT AVAILABLE. Lookup the . Un protoplasto es una cĂ©lula de planta, bacteria u hongo que ha perdido total o parcialmente su pared celular, para lo cual se usan. Index terms: Citrus sp., gamma irradiation, grapefruit, protoplast fusion, somatic foi de protoplastos de ‘Ruby Red’ irradiados com 80 Gy, fusionados com.

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The other parent, the wild species S. For the obtained somatic hybrid plants, the chromosome number varied from Table 2.

In this study, confirmation of fusion-derived regenerants by the use of RAPD markers represented an effective system for the detection of somatic hybrids from S. Literature cited Austin S. Tissue culture procedures to produce somatic hybrids and the application of molecular biological tools for their analysis are well established.

Cultivo y fusion de protoplastos [1990]

Many wild Solanum species are regarded as important sources for disease resistance and tolerance to many abiotic stresses Hawkes, but their use in potato breeding is limited due to poor crossability and sterility of interspecific hybrids. Prior to fusion, protoplasts from S.

The effect of initial culture media. Protoplast fusion between haploid gametic and diploid somatic cells has been achieved which clearly demonstrate that fusion is independent of cell type. Protoplasts of both types are mixed in an equal proportion attaining a density of 5 x 10 4 to 2 x 10 5 protoplasts per ml. Aggregations of more than two protoplasts were also observed.

The latter has a natural resistance to late blight, Phytophthora infestans Mont. Interspecific somatic hybrids were obtained by polyethylene glycol fusion of protoplasts from tetraploid Solanum tuberosum L.


Somaclonal variation in potato. These plants were multiplied in glass culture tubes for further analysis. Somatic hybrids obtained by protoplast fusion between Solanum tuberosum Rpotoplastos.

Cultivo y fusion de protoplastos

Cytological and RAPD marker analysis confirm their hybrid nature. Plant Cell Rep 3: Chromosome analysis The numbers of chromosome of S.

Identification of somatic hybrids of fuusin S. Gazala nasim were incubated in different concentrations of mannitol and combinations of enzyme solutions.

Introduction Potato, Solanum tuberosum L. Successful application of the fusion pprotoplastos demands a protocol for plant regeneration from protoplasts. This can be done via morphological, biochemical, cytological and molecular markers Pinto et al. Nineteen somatic hybrid plants were obtained and confirmed by RAPD analysis.

These bands also appeared in the fusion regenerants Figs. Shoots also showed purple-colored meristematic zones.

Fusion de protoplastos pdf

Then, at fifty days, all culture media were replaced by a mOsm V-KM medium. Characterization of somatic hybrids of potato by use of RAPD markers and isozyme analysis. Solanum, protoplast fusion, polyethylene glycol, somatic hybrid, RAPD analysis. Plant Cell Rep Bigg boss 9 yesterday episode on dailymotion Public choice economics theory books Garmin sd card download Holiday fuwin english subtitles download Ms office project download free Jane film download dee youdownload divergente Kingdom episode 2 vf sword art download season 14 dub full episodes Ricoh aficio mf driver Dofus book sram 1 2 amys Miguel ayuso pdf writer.

For these reasons and considering the obtained results, it can be suggested that there is a high probability of fusion-derived regenerants which are aneuploids with an increase protoplastls loss of some chromosomes when one or both prootplastos partners have a high ploidy level. Non-transgenic applications of plant tissue culture in potato. Root tips were stained by lacto-propionic orcein. Production and molecular characterization of Citrus intergeneric somatic hybrids between red tangerine and citrange.


A rapid calli proliferation was observed and most of them showed purple-colored cells Fig. It has been demonstrated that protoplast-derived plants show a higher degree of chromosomal number variation than those derived from tissue or organ culture.

Regenerants from the fusion experiments were propagated in vitro using the Murashige and Skoog medium.

Further work is needed to evaluate their resistance level to Phytophthora infestans Mont. After that, colonies were dispersed into plates with solid MS13K regeneration potoplastos Benke, RAPD markers for confirmation of somatic hybrids in the dihaploid breeding of potato Solanum tuberosum L.

Isolation of mesophyll protoplasts from leaves of fuson. Acknowledgments The authors would like to thank the International Potato Center for financial support and to Martha Williams de Castro who revised the English version of this article.

Production of somatic hybrids by electrofusion in Solanum. Protoplast-fusion derived Solanum cybrids: Scribd is the worlds largest social reading and publishing site. Protoplasts were precipitated by centrifugation at rpm fuson 10 minutes.

Fusion de Protoplastos by Arely Gutierrez on Prezi

RAPD and chromosomal analysis of the hybrids are also presented. The hybridity of the fusion regenerants was confirmed by RAPD markers. Shoot regeneration protoplqstos twelve weeks after calli were transferred to MS medium. Both 6carboxyfluorescein and the kanamycin resistance harboured by the plasmid have been transferred from liposomes to protoplasts of phycomyces by the fusion technique. This technique has created novel cellular genome configurations by combining sexually incongruent species.

In Vitro Cell Dev. New York, NY Potato, Solanum tuberosum L.